Phosphinothricin-resistant

Author: wgso

August undefined, 2024

WebUnder optimised electroporation conditions, up to 0.9% of viable protoplasts displayed gus activity two days after electroporation. To select for phosphinothricin (PPT) resistant colonies, electroporated protoplasts were incubated for six weeks in a medium containing 10 μg/ml PPT. The cells surviving the selection were maintained as individual ... WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis.

Efficient expression of heterologous genes by the introduction of …

WebPhosphinothricin C5H11NO4P- CID 25244585 - structure, chemical names, physical and … WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) carfentrazone-ethyl in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions … how to share screen hp

Federal Register, Volume 88 Issue 69 (Tuesday, April 11, 2024)

WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1. WebAug 21, 2024 · After 14 days of incubation at 30 °C, carboxin-resistant transformants were screened for phosphinothricin-resistance protein and GUS expression. The transformants expressing opbar were screened on CYM plates with 150 mg/L phosphinothricin. WebPhosphinothricin, as a glutamic acid analog, irreversibly inhibits glutamine synthetase (EC 6.3.1.2) leading to a decrease in the amino acids glutamine, aspartate, serine, glycine, and alanine in the affected organism. The herbicidal effect can be reversed by glutamine (see Bayer et al., 1972 ). notional budget meaning

Resistance to glufosinate is proportional to

Bialaphos - an overview ScienceDirect Topics

WebJan 27, 2024 · The fast spread of bacteria that are resistant to many classes of antibiotics (multidrug resistant) is a global threat to human and animal health with a worrisome scenario ahead. Novel therapeutical strategies are … WebLibertyLink (®) cotton cultivars are engineered for glufosinate resistance by … how to share screen hdmi windowsWebCAS No. 7803-51-2. Phosphine (PH₃) is a colorless, flammable, and explosive gas at room … notional budget definition

"WebA bioassay was developed for identifying transgenic wheat plants based on De Block et al.'s [8] ammonium-multiwell assay which allows qualitative and quantitative evaluation of the expression of the enzyme phosphinothricin acetyl transferase (PAT). Important parameters in the assay are the use of young leaf tissues, short incubation period (6 h) and a high … " - Phosphinothricin-resistant

Phosphinothricin-resistant

Establishment of a chloroplast transformation system in

WebOct 23, 2015 · Increasing PPT-resistant gene diversity and generating multi-herbicide … WebResistance plasmids include pGreenII 0229 and pGreenII 0229 62-SK. pGreenII 0229 is derived from pGreenII 0000, a nos-bar cassette has been inserted into the HpaI site of the left border, providing resistance to bialaphos or phosphinothricin during plant transformation selection. pGreenII 0229 62-SK is derived from pGreenII 0229, the LacZ …

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WebPhosphinothricin is not an effective antibiotic because of its inability to be readily … WebOct 21, 2024 · The CRISPR vector for this study was used in a previously published pBAtC that is composed of the U6 promoter from Arabidopsis to express gRNA including CRISPR RNA scaffold, the 35S promoter originally from cauliflower mosaic virus (CaMV) to express Cas9 and the Basta TM (Phosphinothricin) resistance gene for the selection of the first ...

WebOct 23, 2015 · The R295K mutation has also never been previously reported as an … WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ...

WebWe have previously transformed rice (Oyrza sativa L.) with the maize ribosome-inactivating protein b-32 gene (Zmcrip3a) and the phosphinothricin resistance gene (bar). In the present study, Southern blot hybridization analysis of 56 primary fertile transformants resulted in distinct band patterns, indicating that all the transformants had been generated by … WebJul 14, 2024 · In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene (bar) as a selectable marker was established, and two short peptide-encoding genes (ant1 and ant2) driven by the endogenous psbA and rbcL promoters were cloned in this vector. The recombinant plasmid was transformed using a …

WebThese results indicated that the extra intron at the 5 0 end of the phosphinothricin-resistance gene is required for its efficient expression in G. lucidum. To confirm these results, we also ...

WebA gene which confers resistance to the herbicide bialaphos (bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite. Bialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase. notional budget allocationWebOct 1, 1996 · The enzymes have a similar substrate affinity towards L-phosphinothricin and do not acetylate any of the other L-amino acids tested. ... Engineering herbicide resistance in plants by expression of ... notional building part l notional borrowing costWebresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the how to share screen in facebook live androidWebJan 18, 2024 · One of the microbes that produce phosphinothricin, Streptomyces hygroscopicus, has an enzyme that inactivates phosphinothricin and glufosinate by acylating it. The gene (bar) encoding this... notional budget for schoolsWebNov 6, 2006 · The phosphinothricin-resistant seedlings were uniformly much greener than … how to share screen in discordWebNov 27, 2024 · In the 1980s, the bialaphos resistance ( BAR) gene and its closely related … how to share screen in microsoft teams call